thp 1 bluetm nf κb  (InvivoGen)

Journal: npj Viruses

Article Title: The soluble G protein of respiratory syncytial virus promotes viral dissemination via TLR2-mediated NLRP3 priming and pyroptosis

doi: 10.1038/s44298-026-00172-x

Figure Lengend Snippet: a NF-κB reporter assay in THP-1-Blue cells treated with rsG WT, rsG CX₃Cmut, rBSA, or LTA in the presence or absence of TLR2 or MyD88 inhibitors. SEAP secretion was measured at 550 nm. b Luminex multiplex analysis of IL-6, IL-8, VEGF and CCL2 in A549 cell supernatants after treatment. The data represent the means ± SDs from three independent experiments. Statistical analysis: a Two-way ANOVA with Tukey’s post hoc test. b Kruskal‒Wallis test with Dunn’s post hoc test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: THP-1 blue NF-κB cells (InvivoGen) were seeded in 96-well plates and treated with 50 or 150 nM rBSA, rsG WT, or the rsG CX3C mutant, with or without 200 nM C29 (MedChemExpress HY-100461) or 50 nM TJ-M2010-5 (MedChemExpress HY-139397) for 1 h. After 18 h, SEAP activity was measured by transferring the supernatant to Quanti-BlueTM reagent (InvivoGen) and reading the absorbance at 650 nm after 2 h.

Techniques: Reporter Assay, Luminex, Multiplex Assay

Journal: npj Viruses

Article Title: The soluble G protein of respiratory syncytial virus promotes viral dissemination via TLR2-mediated NLRP3 priming and pyroptosis

doi: 10.1038/s44298-026-00172-x

Figure Lengend Snippet: a RT‒qPCR analysis of NLRP3 mRNA expression in A549 cells after treatment with sG, rBSA, RSV or LTA at the indicated time points. b Caspase-1 activity in A549 cells pretreated with sG and superinfected with RSV, with or without the NLRP3 inhibitor MCC950. c Western blot detection of full-length and cleaved gasdermin D (GSDMD) in cell lysates. Lanes 1–4 and 5–8 represent untreated (1/5), rBSA 150 nM (2/6), rsG WT (3/7) and rsG CX3Cmut (4/8). Lane 9 represents in both panels (left (L)/right (R)) a PMA + LPS-treated THP-1 positive control. a , b Data represent the mean ± SD from three independent experiments. Statistical analysis: two-way ANOVA with Tukey’s post hoc test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: THP-1 blue NF-κB cells (InvivoGen) were seeded in 96-well plates and treated with 50 or 150 nM rBSA, rsG WT, or the rsG CX3C mutant, with or without 200 nM C29 (MedChemExpress HY-100461) or 50 nM TJ-M2010-5 (MedChemExpress HY-139397) for 1 h. After 18 h, SEAP activity was measured by transferring the supernatant to Quanti-BlueTM reagent (InvivoGen) and reading the absorbance at 650 nm after 2 h.

Techniques: Expressing, Activity Assay, Western Blot, Positive Control

Journal: npj Viruses

Article Title: The soluble G protein of respiratory syncytial virus promotes viral dissemination via TLR2-mediated NLRP3 priming and pyroptosis

doi: 10.1038/s44298-026-00172-x

Figure Lengend Snippet: a Western blot analysis of TLR2 and CX3CR1 expression in the indicated cell lines. b GFP fluorescence imaging of A549, HEp-2, HULEC-5a, THP-1 and U937 cells after RSV-A-0594-eGFP infection. c Quantification of LDH release into the supernatants of RSV-A-0594-infected recombinant protein-treated cells treated with or without the NLRP3 inhibitor MCC950. The data represent the means ± SDs from three independent experiments. Statistical analysis: linear regression and two-way ANOVA with Tukey’s post hoc test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: THP-1 blue NF-κB cells (InvivoGen) were seeded in 96-well plates and treated with 50 or 150 nM rBSA, rsG WT, or the rsG CX3C mutant, with or without 200 nM C29 (MedChemExpress HY-100461) or 50 nM TJ-M2010-5 (MedChemExpress HY-139397) for 1 h. After 18 h, SEAP activity was measured by transferring the supernatant to Quanti-BlueTM reagent (InvivoGen) and reading the absorbance at 650 nm after 2 h.

Techniques: Western Blot, Expressing, Fluorescence, Imaging, Infection, Recombinant